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SlZF3基因参与植物耐盐性

2017-12-05来源:莱肯生物
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      非生物胁迫是造成作物产量损失的重要因素。抗坏血酸也称Vc通过清除植物在响应非生物胁迫时产生的大量活性氧(ROS)而增强植物的胁迫耐性。尽管Vc的合成和代谢路径已经很清晰,然而该途径中的基因调控机制尚未明确。近日,华中农业大学的欧阳波博士报道了番茄中的一个编码含有EAR转录抑制基序的 Cys2/His2-型锌指蛋白——SlZF3基因参与Vc代谢途径的调控模式。SlZF3基因在盐胁迫条件下迅速上调表达。过表达SlZF3基因的转基因番茄和拟南芥Vc含量显著升高,进而Vc介导的ROS清除能力增强,耐盐性增强。蛋白质相互作用的研究结果表明,SlZF3蛋白与COP9信号转导体关键元件CSN5B蛋白直接结合,二者的结合抑制了CSN5B蛋白与VTC1蛋白的结合,从而阻止CSN5B对VTC1的降解,后者是参与Vc生物合成的GDP-Man焦磷酸化酶。研究发现EAR基序能够促进SlZF3蛋白的稳定性,而对于SlZF3和CSN5B蛋白的互作则不是必须的。该研究表明SlZF3在促进Vc积累的同时提高植物的耐盐性。
Plant Biotechnology Journal, 28 November 2017
The C2H2 zincfinger-protein SlZF3 regulates AsA synthesis and salt tolerance by interactingwith CSN5B
Authors
Ying Li,Zhuannan Chu,Jinying Luo,Yuhong Zhou,Yujing Cai,YongenLu,Junhui Xia,Hanhui Kuang,Zhibiao Ye,Bo Ouyang
Summary
Abiotic stresses are a major cause of crop loss. Ascorbic acid (AsA) promotes stress tolerance by scavenging reactive oxygen species (ROS), which accumulate when plants experience abiotic stress. Although the biosynthesis and metabolism of AsA are well established, the genes that regulate these pathways remain largely unexplored. Here, we report on a novel regulatory gene from tomato (Solanum lycopersicum) named SlZF3 that encodes a Cys2/His2-type zinc-finger protein with an EAR repression domain. The expression of SlZF3 was rapidly induced by NaCl treatments. The overexpression of SlZF3 significantly increased the levels of AsA in tomato and Arabidopsis. Consequently, the AsA-mediated ROS scavenging capacity of the SlZF3-overexpressing plants was increased, which enhanced the salt tolerance of these plants. Protein-protein interaction assays demonstrated that SlZF3 directly binds CSN5B, a key component of the COP9 signalosome. This interaction inhibited the binding of CSN5B to VTC1, a GDP-Man pyrophosphorylase that contributes to AsA biosynthesis. We found that the EAR domain promoted the stability of SlZF3 but was not required for the interaction between SlZF3 and CSN5B. Our findings indicate that SlZF3 simultaneously promotes the accumulation of AsA and enhances plant salt-stress tolerance.

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