当前位置:  农事资讯热点话题科技知产

高抗草甘膦水稻的培育

2017-06-04来源:莱肯生物
分享到: 更多
   切换手机版

      耐草甘膦转基因作物可以通过三种方式获得:

      1、超量表达EPSPS蛋白,补偿草甘膦造成的EPSPS蛋白损失;

      2、转入epsps和aroA等基因,产生对草甘膦不敏感的EPSPS蛋白;

      3、转入草甘膦-N-乙酰转移酶GAT,直接使草甘膦失活。其中,利用第二种方法开发的转基因作物草甘膦抗性效果最好,因此应用最广泛。代表性的产品为孟山都公司利用农杆菌cp4菌株中的cp4 epsps基因开发的转基因玉米NK603和转基因大豆40-3-2。

      cp4 epsps基因的专利保护权已于2014年过期(US 5633435 A),可以用其开发新的转化体。但是,过渡依赖单一类型的抗性基因可能造成“抗性杂草”的产生。近年来,国内多家单位开发出了很多新型的草甘膦抗性基因,如:假单胞菌株G2、G6、G10中克隆的epsps和aroA等。为未来耐草甘膦转基因作物的培育扩展了基因来源。
      最近,中国农科院生物技术所王志兴和王旭静团队利用G2-EPSPS基因培育了可耐受田间推荐浓度20倍的抗草甘膦转基因水稻及事件特异性的检测方法。

Front. Plant Sci., 30 May 2017
Development and Event-specific Detection of Transgenic Glyphosate-resistant Rice Expressing the G2-EPSPS Gene
Author
Yufeng Dong, Xi Jin, Qiaoling Tang,et al.
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, China
Abstract
Glyphosate is a widely used herbicide, due to its broad spectrum, low cost, low toxicity, high efficiency, and non-selective characteristics. Rice farmers rarely use glyphosate as a herbicide, because the crop is sensitive to this chemical. The development of transgenic glyphosate-tolerant rice could greatly improve the economics of rice production. Here, we transformed the Pseudomonas fluorescens G2 5-enolpyruvyl shikimate-3-phosphatesynthase (EPSPS) gene G2-EPSPS, which conferred tolerance toglyphosate herbicide into a widely used japonica rice cultivar, Zhonghua 11 (ZH11), to develop two highly glyphosate-tolerant transgenic rice lines, G2-6 and G2-7, with one exogenous gene integration. Seed germination tests and glyphosate-tolerance assays of plants grown in a greenhouse showed that the two transgenic lines could greatly improve glyphosate-tolerance compared with the wild-type; The glyphosate-tolerance field test indicated that both transgenic lines could grow at concentrations of 20,000 ppm glyphosate, which is more than 20-times the recommended concentration in the field. Isolation of the flanking sequence of transgenic rice G2-6 indicated that the 5′-terminal of T-DNA was inserted into chromosome 8 of the rice genome. An event-specific PCR test system was established and the limit of detection of the primers reached five copies. Overall, the G2-EPSPS gene significantly improved glyphosate-tolerance in transgenic rice; furthermore, it is a useful candidategene for the future development of commercial transgenic rice.


图文来源网络 如有侵权 请联系删除

扫描二维码关注智农361公众号,了解更多农事资讯

手机长按二维码识别

[责任编辑:wangjing] 标签: nbsp 草甘膦 转基因 基因 抗性
您可能喜欢的

友情链接

微信分享